您的位置：首頁 > 技術文章 > 微生物 細菌凍存方法
更新時間：2013-05-26 瀏覽次數：1684
Bacterium preparationCryoprotective agentTrueCoolTM cryovial
CoolBoxTM CFT30 ice-free cooling stationCoolRack® CFT30CoolRack® CFCryolabels and/or cryomarkersThermalTrayHP platform （optional）CoolSinkTM BX50 （optional）37°C waterbath-80°C Freezer
1. Bacteria PreparationFollow the laboratory protocol for bacterial growth and preparation.Refer to CDC （Centers for Disease Control and Prevention） guidelines for utilization of pathogens in specific BSL （Biosafety Level）. Pathogens are infectious agents and should be manipulated in a controlled and safe environment.
2. Bacteria Freezing1） As a general rule, maintain the bacterial preparation at 4°C by placing it in a boat-reservoir in CoolSinkBX50 and/or ThermalTray on ice.
2） Prepare a bacteria glycerol stock by diluting the bacterial preparation with a sterile glycerol solution for a final 15-50% v/v glycerol concentration. Dispense 1 ml of stock （or desired amount） in a pre-labeled TrueCool cryovial. To avoid titer reduction maintain the vials on a CoolRackCFT30 in the CoolBox at 4°C. The CoolRackCFT30 reduces contamination and spill accidents by allowing one-handed opening/closing of the cryovials.
3） While bacteria samples are kept cold at 4°C in the CoolRackCFT30, equilibrate a CoolRackCF on dry-ice for 10 minutes. Note: with this protocol, there is no need to make a dry-ice/ethanol slurry. Place the cryotubes directly on the pre-equilibrated CoolRackCF and snap-freeze the samples for 3-5 minutes.
4） Transfer the frozen samples to a storage box and place it in the -80°C freezer for long-term storage.
3. Bacteria Thawing1） Place the cryovials from the -80°C freezer in a CoolBoxCFT30 with a -12°C frozen cartridge inside to maintain the vials at the correct temperature and allow transport of the vials in a safe manner.
2） Place the vials directly in a 37°C water bath, and manually slowly agitate the vials to enable the thawing process. Just before the whole liquid is compley thawed, remove the vial from the 37°C water bath and place it on a CoolRackCFT30 previously equilibrated on ice. Samples are ready for experimental procedures or titer assessment.
首頁|公司簡介|資質證書|產品中心|技術文章|公司新聞|聯系我們|GoogleSitemap
聯系人：周經理 劉經理 郵箱：beinuobio@163.com
管理登陸 技術支持：化工儀器網
主營產品：免疫學，細胞學，分子生物學，微生物學，即用型試劑，常規生化試劑一站式供應平臺,生命科學領域優質產品和服務供應商
© 2025 上海北諾生物科技有限公司(www.fsbhkies.cn) 版權所有 備案號： 總訪問量：1010198